ResultsĪ remarkable remineralization of WSLs in SEM images was observed in all treated groups compared to CTRL. The surface micromorphology (SEM), the chemical/elemental composition (RMS and EDS), and the Vickers microhardness (VMH) were evaluated. Teeth samples were submitted to the following protocol: a 7-day pH cycling treatment, with two daily exposures (2 min each time) to the commercial toothpastes described above. All toothpastes tested contained 1450 ppm of fluoride. N = 12 human molars with natural WSLs in the proximal-vestibular zone were selected and divided into 4 groups ( n = 3) according to the different treatments: HAF (hydroxyapatite with fluoride ions) SMF (sodium monofluorophosphate with arginine) SF (sodium fluoride with enzymes), and CTRL (untreated group). A multidisciplinary approach based on Raman microspectroscopy (RMS), Scanning electron microscopy (SEM), Energy-dispersive x-ray spectroscopy (EDS), and Vickers microhardness (VMH) was exploited. This in vitro study aimed assessing the remineralization potential of three commercial fluoride-based toothpastes in permanent teeth with natural white spot lesions (WSLs).
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